ck2 inhibitor silmitasertib cx 4945  (MedChemExpress)

Journal: Islets

Article Title: CK2 regulates somatostatin expression in pancreatic delta cells

doi: 10.1080/19382014.2025.2515332

Figure Lengend Snippet: Expression of CK2 in RIN14B cells. (A) bright field images showing the morphology of RIN14B cells (scale bar in left panel: 800 µm; scale bar in right panel: 200 µm). (B) Representative Western blots of β-actin, PDX1, CK2α, CK2β expression in whole cell extracts of RIN14B cells. (C) Representative immunofluorescence stainings of CK2α (green, upper panel) and PDX1 (green, lower panel) in RIN14B cells. Cell nuclei were stained with Hoechst 33,342 (blue). Scale bar: 50 µm.

Article Snippet: The CK2 inhibitors CX-4945 and SGC (SGC-CK2-1) were purchased from SelleckChem (Munich, Germany).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining

Journal: Islets

Article Title: CK2 regulates somatostatin expression in pancreatic delta cells

doi: 10.1080/19382014.2025.2515332

Figure Lengend Snippet: CK2 inhibition does not affect viability and SST expression in RIN14B cells. (A) Representative Western blots of Akt, pAkt, β-actin, CK2α and CK2β expression in whole cell extracts of RIN14B cells exposed to CX-4945, SGC or vehicle for 24 h. (B and C) RIN14B cells were treated as described in (A) and the viability was analyzed by a WST-1 assay (B) and LDH assay (C). Data are expressed in % of vehicle ( n = 3 each). Mean ± SD. (D) Quantitative analysis of SST mRNA expression in RIN14B cells treated as described in (A). Data are expressed in % of vehicle ( n = 3 each). Mean ± SD. (E) Representative immunofluorescence stainings of RIN14B cells overexpressing FLAG-tagged PDX1-WT. FLAG-Tag (red, upper left panel) and PDX1 (green, upper right panel) in RIN14B cells. Cell nuclei were stained with Hoechst 33,342 (blue). Scale bar: 25 µm. (F) Quantitative analysis of SST mRNA expression in RIN14B cells overexpressing FLAG-tagged PDX1-WT or control vector (mock). Data are expressed in % of mock ( n = 3 each). Mean ± SD.

Article Snippet: The CK2 inhibitors CX-4945 and SGC (SGC-CK2-1) were purchased from SelleckChem (Munich, Germany).

Techniques: Inhibition, Expressing, Western Blot, WST-1 Assay, Lactate Dehydrogenase Assay, Immunofluorescence, FLAG-tag, Staining, Control, Plasmid Preparation

Journal: Islets

Article Title: CK2 regulates somatostatin expression in pancreatic delta cells

doi: 10.1080/19382014.2025.2515332

Figure Lengend Snippet: CK2 inhibition increases SST expression and secretion in murine isolated islets. (A) Schematic illustration of the experimental setting: islets were isolated from mice and exposed to CX-4945, SGC or vehicle for 24 h. Subsequently, the islets were collected and prepared for protein expression and secretion analyses. (B) Representative immunofluorescence stainings of CK2α (green) and SST (red) in isolated murine islets. Cell nuclei were stained with Hoechst 33,342 (blue). Scale bar: 100 µm. (C) Isolated murine islets were treated as described in (A) and the number of SST-positive cells was determined in % of vehicle-treated islets. Mean ± SD. (D) Representative Western blots of Akt, pAkt and β-actin in whole cell extracts of murine islets treated as described in (A). (E) Murine islets were treated as described in (A) and SST mRNA expression was assessed. Data are expressed in % of vehicle ( n = 3 each). Mean ± SD. * p < 0.05. (F) Murine islets were treated as described in (A) and SST secretion was quantitatively analyzed (ng/mL) ( n = 3 each). Mean ± SD. * p < 0.05.

Article Snippet: The CK2 inhibitors CX-4945 and SGC (SGC-CK2-1) were purchased from SelleckChem (Munich, Germany).

Techniques: Inhibition, Expressing, Isolation, Immunofluorescence, Staining, Western Blot

Journal: Islets

Article Title: CK2 regulates somatostatin expression in pancreatic delta cells

doi: 10.1080/19382014.2025.2515332

Figure Lengend Snippet: CK2 inhibition increases SST expression under physiological conditions. (A) Schematic illustration of the experimental setting: mice were treated with CX-4945 or vehicle. After 3 d, the mice were euthanized and islets as well as plasma were isolated to study SST gene expression, insulin and glucagon plasma levels. (B and C) Quantitative analysis of plasma insulin levels (pmol/L) and plasma glucagon levels (ng/L) of mice treated as described in (A) ( n = 3 each). Mean ± SD. * p < 0.05. (D) SST mRNA expression of isolated islets from mice treated as described in (A). Data are expressed in % of vehicle ( n = 3 each). Mean ± SD. * p < 0.05. (E) Schematic illustration of the experimental setting: islets were isolated from pancreata of human donors. The islets were then exposed to CX-4945, SGC or vehicle and the subsequent SST secretion was assessed. (F) Human islets were treated as described in (E) and SST secretion was quantitatively analyzed (ng/mL) ( n = 2 each). Mean ± SD.

Article Snippet: The CK2 inhibitors CX-4945 and SGC (SGC-CK2-1) were purchased from SelleckChem (Munich, Germany).

Techniques: Inhibition, Expressing, Clinical Proteomics, Isolation, Gene Expression